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direct dna sequencing  (Thermo Fisher)


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    Structured Review

    Thermo Fisher direct dna sequencing
    <t>DNA</t> <t>sequencing</t> profiles of the heterozygous δ −276(A>G) (A) and the homozygous δ −77(T>C) (B).
    Direct Dna Sequencing, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/direct+dna+sequencing/Deoxyribonucleic+acid/pmc12229147-53-0-12
    Average 99 stars, based on 1 article reviews
    direct dna sequencing - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Molecular and hematological characteristics of two different δ-globin promoter variants, δ −276(A>G) and δ −77(T >C) among Thai, Burmese, and Laotian subjects"

    Article Title: Molecular and hematological characteristics of two different δ-globin promoter variants, δ −276(A>G) and δ −77(T >C) among Thai, Burmese, and Laotian subjects

    Journal: PeerJ

    doi: 10.7717/peerj.19636

    DNA sequencing profiles of the heterozygous δ −276(A>G) (A) and the homozygous δ −77(T>C) (B).
    Figure Legend Snippet: DNA sequencing profiles of the heterozygous δ −276(A>G) (A) and the homozygous δ −77(T>C) (B).

    Techniques Used: DNA Sequencing

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Epigenetic silencing of the tumor suppressor TIMP-3 gene in upstream CpG islands in thyroid neoplasms: a cross-sectional study with systematic review.
    Article Snippet: Background Despite advances in understanding thyroid cancer pathogenesis, the specific epigenetic mechanisms driving malignant transformation remain incompletely characterized.. While genetic variations have been well documented, the role of DNA methylation, particularly involving tumor suppressor genes, represents a critical knowledge gap.. This resaerch aimed to evaluate the methylation status of the tissue inhibitor of metalloproteinase 3 (TIMP-3) promoter across 15 CpG sites in papillary and follicular thyroid carcinomas (PTC and FTC) compared to benign thyroid lesions, and to contextualize these findings through a review of previous studies on TIMP-3 promoter methylation.

    DNA Sequencing:

    Article Title: Epigenetic silencing of the tumor suppressor TIMP-3 gene in upstream CpG islands in thyroid neoplasms: a cross-sectional study with systematic review.
    Article Snippet: Background Despite advances in understanding thyroid cancer pathogenesis, the specific epigenetic mechanisms driving malignant transformation remain incompletely characterized.. While genetic variations have been well documented, the role of DNA methylation, particularly involving tumor suppressor genes, represents a critical knowledge gap.. This resaerch aimed to evaluate the methylation status of the tissue inhibitor of metalloproteinase 3 (TIMP-3) promoter across 15 CpG sites in papillary and follicular thyroid carcinomas (PTC and FTC) compared to benign thyroid lesions, and to contextualize these findings through a review of previous studies on TIMP-3 promoter methylation.

    Article Title: Prevalence and Molecular Characterization of Cryptosporidium Species in Diarrheic Children in Cameroon.
    Article Snippet: Amplicons were subsequently sent for sequencing (Inqaba Biotec, Pretoria, South Africa). .. Direct DNA sequencing of GP60 amplicons purified from the gels was performed using the primers GP60-AL3532 (5′-TCCGCTGTATTCTCAGCC-3′) and GP60-AL3534 (5′- GCAGAGGAACCAGCATC-3′) on an ABI PRISM—3730 XL DNA Analyzer using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genetic polymorphism of novel SNP rs5006884 in OR51B6 and SNP rs4499252 in AHSP among transfusion-dependent and non-transfusion-dependent β-thalassemia/Hb E patients in Thailand: a multivariate analysis of clinical and genetic polymorphism
    Article Snippet: .. Whereas rare β-thalassemia mutations were further analyzed with samples that were not found to contain common mutations, using direct DNA sequencing on an ABI PRISMTM 3130 XLanalyzer (Applied Biosystems, Foster City, CA, USA), as described [ ]. ..

    Article Title: Unraveling thalassemia intermedia: Novel insights of a hemoglobin Jax [HBA2:c.44G>C] and deletional α 0 -thalassemia interaction phenotype.
    Article Snippet: .. Selective amplification of a1- and a2-globin genes was performed according to the protocol by Dod e et al.21 After amplification, the products were purified using a GF-1 AmbiClean kit (Vivantis Technologies), and direct DNA sequencing was conducted using an ABI PRISM 3130xl automated analyzer (Applied Biosystems, Foster City, California, USA). a-globin haplotype analysis To demonstrate the evolution and origin of Hb Jax, the a-globin gene haplotypes were determined using seven common polymorphisms. ..

    Article Title: Unraveling thalassemia intermedia: Novel insights of a hemoglobin Jax [HBA2:c.44G>C] and deletional α 0 -thalassemia interaction phenotype
    Article Snippet: .. Selective amplification of α 1 - and α -globin genes was performed according to the protocol by Dodé et al. After amplification, the products were purified using a GF-1 AmbiClean kit (Vivantis Technologies), and direct DNA sequencing was conducted using an ABI PRISM 3130xl automated analyzer (Applied Biosystems, Foster City, California, USA). ..

    Article Title: Molecular and hematological characteristics of two different δ-globin promoter variants, δ −276(A>G) and δ −77(T >C) among Thai, Burmese, and Laotian subjects
    Article Snippet: .. Direct DNA sequencing of the whole δ-globin gene was done on an ABI PRISMTM 3730 XL analyzer (Applied Biosystems, Foster City, CA, USA) or Barcode-tagged sequencing (BTSeqTM, Celemics, Korea). ..

    Article Title: Prevalence and Molecular Characterization of Cryptosporidium Species in Diarrheic Children in Cameroon
    Article Snippet: Amplicons were subsequently sent for sequencing (Inqaba Biotec, Pretoria, South Africa). .. Direct DNA sequencing of GP60 amplicons purified from the gels was performed using the primers GP60-AL3532 (5′-TCCGCTGTATTCTCAGCC-3′) and GP60-AL3534 (5′-GCAGAGGAACCAGCATC-3′) on an ABI PRISM—3730 XL DNA Analyzer using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Genetic polymorphism of novel SNP rs5006884 in OR51B6 and SNP rs4499252 in AHSP among transfusion-dependent and non-transfusion-dependent β-thalassemia/Hb E patients in Thailand: a multivariate analysis of clinical and genetic polymorphism.
    Article Snippet: .. Whereas rare β-thalassemia mutations were further analyzed with samples that were not found to contain common mutations, using direct DNA sequencing on an ABI PRISMTM 3130 XLanalyzer (Applied Biosystems, Foster City, CA, USA), as described [12]. ..

    Methylation:

    Article Title: Epigenetic silencing of the tumor suppressor TIMP-3 gene in upstream CpG islands in thyroid neoplasms: a cross-sectional study with systematic review.
    Article Snippet: Background Despite advances in understanding thyroid cancer pathogenesis, the specific epigenetic mechanisms driving malignant transformation remain incompletely characterized.. While genetic variations have been well documented, the role of DNA methylation, particularly involving tumor suppressor genes, represents a critical knowledge gap.. This resaerch aimed to evaluate the methylation status of the tissue inhibitor of metalloproteinase 3 (TIMP-3) promoter across 15 CpG sites in papillary and follicular thyroid carcinomas (PTC and FTC) compared to benign thyroid lesions, and to contextualize these findings through a review of previous studies on TIMP-3 promoter methylation.

    Purification:

    Article Title: Prevalence and Molecular Characterization of Cryptosporidium Species in Diarrheic Children in Cameroon.
    Article Snippet: Amplicons were subsequently sent for sequencing (Inqaba Biotec, Pretoria, South Africa). .. Direct DNA sequencing of GP60 amplicons purified from the gels was performed using the primers GP60-AL3532 (5′-TCCGCTGTATTCTCAGCC-3′) and GP60-AL3534 (5′- GCAGAGGAACCAGCATC-3′) on an ABI PRISM—3730 XL DNA Analyzer using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Unraveling thalassemia intermedia: Novel insights of a hemoglobin Jax [HBA2:c.44G>C] and deletional α 0 -thalassemia interaction phenotype.
    Article Snippet: .. Selective amplification of a1- and a2-globin genes was performed according to the protocol by Dod e et al.21 After amplification, the products were purified using a GF-1 AmbiClean kit (Vivantis Technologies), and direct DNA sequencing was conducted using an ABI PRISM 3130xl automated analyzer (Applied Biosystems, Foster City, California, USA). a-globin haplotype analysis To demonstrate the evolution and origin of Hb Jax, the a-globin gene haplotypes were determined using seven common polymorphisms. ..

    Article Title: Unraveling thalassemia intermedia: Novel insights of a hemoglobin Jax [HBA2:c.44G>C] and deletional α 0 -thalassemia interaction phenotype
    Article Snippet: .. Selective amplification of α 1 - and α -globin genes was performed according to the protocol by Dodé et al. After amplification, the products were purified using a GF-1 AmbiClean kit (Vivantis Technologies), and direct DNA sequencing was conducted using an ABI PRISM 3130xl automated analyzer (Applied Biosystems, Foster City, California, USA). ..

    Article Title: Prevalence and Molecular Characterization of Cryptosporidium Species in Diarrheic Children in Cameroon
    Article Snippet: Amplicons were subsequently sent for sequencing (Inqaba Biotec, Pretoria, South Africa). .. Direct DNA sequencing of GP60 amplicons purified from the gels was performed using the primers GP60-AL3532 (5′-TCCGCTGTATTCTCAGCC-3′) and GP60-AL3534 (5′-GCAGAGGAACCAGCATC-3′) on an ABI PRISM—3730 XL DNA Analyzer using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). ..

    Sequencing:

    Article Title: Prevalence and Molecular Characterization of Cryptosporidium Species in Diarrheic Children in Cameroon.
    Article Snippet: Amplicons were subsequently sent for sequencing (Inqaba Biotec, Pretoria, South Africa). .. Direct DNA sequencing of GP60 amplicons purified from the gels was performed using the primers GP60-AL3532 (5′-TCCGCTGTATTCTCAGCC-3′) and GP60-AL3534 (5′- GCAGAGGAACCAGCATC-3′) on an ABI PRISM—3730 XL DNA Analyzer using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Molecular and hematological characteristics of two different δ-globin promoter variants, δ −276(A>G) and δ −77(T >C) among Thai, Burmese, and Laotian subjects
    Article Snippet: .. Direct DNA sequencing of the whole δ-globin gene was done on an ABI PRISMTM 3730 XL analyzer (Applied Biosystems, Foster City, CA, USA) or Barcode-tagged sequencing (BTSeqTM, Celemics, Korea). ..

    Article Title: Prevalence and Molecular Characterization of Cryptosporidium Species in Diarrheic Children in Cameroon
    Article Snippet: Amplicons were subsequently sent for sequencing (Inqaba Biotec, Pretoria, South Africa). .. Direct DNA sequencing of GP60 amplicons purified from the gels was performed using the primers GP60-AL3532 (5′-TCCGCTGTATTCTCAGCC-3′) and GP60-AL3534 (5′-GCAGAGGAACCAGCATC-3′) on an ABI PRISM—3730 XL DNA Analyzer using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). ..

    Amplification:

    Article Title: Unraveling thalassemia intermedia: Novel insights of a hemoglobin Jax [HBA2:c.44G>C] and deletional α 0 -thalassemia interaction phenotype.
    Article Snippet: .. Selective amplification of a1- and a2-globin genes was performed according to the protocol by Dod e et al.21 After amplification, the products were purified using a GF-1 AmbiClean kit (Vivantis Technologies), and direct DNA sequencing was conducted using an ABI PRISM 3130xl automated analyzer (Applied Biosystems, Foster City, California, USA). a-globin haplotype analysis To demonstrate the evolution and origin of Hb Jax, the a-globin gene haplotypes were determined using seven common polymorphisms. ..

    Article Title: Unraveling thalassemia intermedia: Novel insights of a hemoglobin Jax [HBA2:c.44G>C] and deletional α 0 -thalassemia interaction phenotype
    Article Snippet: .. Selective amplification of α 1 - and α -globin genes was performed according to the protocol by Dodé et al. After amplification, the products were purified using a GF-1 AmbiClean kit (Vivantis Technologies), and direct DNA sequencing was conducted using an ABI PRISM 3130xl automated analyzer (Applied Biosystems, Foster City, California, USA). ..



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    Image Search Results


    DNA sequencing profiles of the heterozygous δ −276(A>G) (A) and the homozygous δ −77(T>C) (B).

    Journal: PeerJ

    Article Title: Molecular and hematological characteristics of two different δ-globin promoter variants, δ −276(A>G) and δ −77(T >C) among Thai, Burmese, and Laotian subjects

    doi: 10.7717/peerj.19636

    Figure Lengend Snippet: DNA sequencing profiles of the heterozygous δ −276(A>G) (A) and the homozygous δ −77(T>C) (B).

    Article Snippet: Direct DNA sequencing of the whole δ-globin gene was done on an ABI PRISMTM 3730 XL analyzer (Applied Biosystems, Foster City, CA, USA) or Barcode-tagged sequencing (BTSeqTM, Celemics, Korea).

    Techniques: DNA Sequencing